Sequence-imposed structural constraints in the TonB protein ofE. coli
نویسندگان
چکیده
منابع مشابه
On the Constraints Imposed
The respectively construction in English is exempliied by sentence (1): (1) English and Dutch are used in Guyana and Suriname, respectively. Gr] Example (1) establishes two relations: one between English and Guyana, and one between Dutch and Suriname. Such examples have often been taken to show that respectively induces an intrasentential syntactic dependency among corresponding members of two ...
متن کاملIdentification of the Escherichia coli tonB gene product in minicells containing tonB hybrid plasmids.
Sotlillm clodtq~l sul~~t~e/polyacrylarnide gel :tnwlysis ot’ protrins t~codcd by ii sc>rics ot’ to?/ R + plasmids in minicells has icknt~ified the torLB gene product ws a prokin wit)h km apparent, molecular weight of’ 36,000. A parallel analysis of s(‘vcn tort N mnt,ations which have been genetically crosst~i onto a tonB+ plasmid support,s this itkntification; t)he 36,000 M, protein is tlbsent ...
متن کاملConfined Mobility of TonB and FepA in Escherichia coli Membranes
The important process of nutrient uptake in Escherichia coli, in many cases, involves transit of the nutrient through a class of beta-barrel proteins in the outer membrane known as TonB-dependent transporters (TBDTs) and requires interaction with the inner membrane protein TonB. Here we have imaged the mobility of the ferric enterobactin transporter FepA and TonB by tracking them in the membran...
متن کاملConstraints imposed by non-functional protein–protein interactions on gene expression and proteome size
Crowded intracellular environments present a challenge for proteins to form functional specific complexes while reducing non-functional interactions with promiscuous non-functional partners. Here we show how the need to minimize the waste of resources to non-functional interactions limits the proteome diversity and the average concentration of co-expressed and co-localized proteins. Using the r...
متن کاملtonB-independent ferrichrome-mediated iron transport in Escherichia coli spheroplasts.
Although a functional tonB gene product was required for ferrichrome-mediated iron transport in whole cells of Escherichia coli K-12, such transport did not require the tonB+ function in spheroplasts. We suggest that in spheroplasts ferrichrome has direct access to the cytoplasmic membrane and that this is reflected in tonB-independent accumulation of ferrichrome iron. Therefore, the tonB gene ...
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ژورنال
عنوان ژورنال: FEBS Letters
سال: 1986
ISSN: 0014-5793
DOI: 10.1016/0014-5793(86)81020-1